Maciej Tworek studied piano with Jan Hoffman, and choral studies and conducting with Józef Radwan at the Academy of Music in Kraków. He also attended masterclasses with Helmuth Rilling, Roger Norrington, and Kurt Masur, and worked as an assistant to Valery Gergiev and Lorin Maazel. Conductor of Capella Cracoviensis in 2005–12, he currently teaches at the Krzysztof Penderecki Academy of Music in Kraków.
Since 2002, he has worked closely with Krzysztof Penderecki, recording his symphonies and concertos with the Jerzy Semkow Polish Sinfonia Iuventus Orchestra. In 2018, he conducted the Rundfunk-Sinfonieorchester Berlin during the Berlin performance of the Violin Concerto No. 2 Metamorphosen, with soloist Anne-Sophie Mutter, before embarking on a tour of China with Sinfonia Varsovia. In 2019, he conducted A Polish Requiem in the presence of the presidents of Poland and Germany and the Vice President of the United States, and then the Korean premiere of St Luke Passion.
He has conducted ensembles and orchestras including The Hilliard Ensemble, the NDR Radiophilharmonie, the MDR-Sinfonieorchester, Tonhalle Zürich, the Dresdner Philharmonie, the Tokyo Metropolitan Symphony Orchestra, and the Jerusalem Symphony Orchestra, in Europe, Asia, and the Americas.